recombinant serca2 protein (Novus Biologicals)
Structured Review

Recombinant Serca2 Protein, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+serca2+protein/Recombinant+Human+SERCA2+ATPase+GST+(N-Term)+Protein/pm39905176-230-13-18
Average 93 stars, based on 1 article reviews
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1) Product Images from "The Sarcoplasmic/Endoplasmic reticulum Ca 2+ -ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation."
Article Title: The Sarcoplasmic/Endoplasmic reticulum Ca 2+ -ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation.
Journal: Scientific reports
doi: 10.1038/s41598-025-88012-5
Figure Legend Snippet: Fig. 1. Presence and localization of SERCA2 in pig sperm. (a) Representative immunoblotting of SERCA2 in pig sperm after incubation with the anti-SERCA2 antibody. MW: Molecular Weight; 1–3 correspond to different samples. The black box indicates the SERCA2 band. (b) Representative immunofluorescence analysis of SERCA2 in pig sperm. Sperm nuclei are shown in blue (DAPI), whereas SERCA2 is shown in green. The white arrow shows the localization of SERCA2 in the midpiece, whereas arrowheads show its presence in the acrosome. Scale bars: 20 μm.
Techniques Used: Western Blot, Incubation, Molecular Weight, Immunofluorescence
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Competitive Binding Assay:Article Title: The Sarcoplasmic/Endoplasmic reticulum Ca 2+ -ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation. Article Snippet: Thereafter, membranes were washed thrice in 1× TBS-Tween 20, and incubated with a secondary goat anti-rabbit antibody (ref. P0448; Dako; 1:10,000; v: v) in agitation for 1 h. Finally, membranes were washed five times in 1× TBS-Tween 20 and bands were visualized using a chemiluminescent substrate in a G: BOX Chemi XL system (Syngene). .. To confirm the specificity of the primary antibody, a competition assay using a Article Title: The Sarcoplasmic/Endoplasmic reticulum Ca 2+ -ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation Article Snippet: Samples were observed under a confocal laser-scanning microscope (CLSM, Nikon A1R; Nikon Corp., Tokyo, Japan) with preset acquisition settings, and the images captured were analyzed with the Fiji ImageJ software . .. To confirm the primary antibody’s specificity, a competition assay was performed using a Article Title: The Sarcoplasmic/Endoplasmic reticulum Ca 2+ -ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation. Article Snippet: Samples were observed under a confocal laser-scanning microscope (CLSM, Nikon A1R; Nikon Corp., Tokyo, Japan) with preset acquisition settings, and the images captured were analyzed with the Fiji ImageJ software57. .. To confirm the primary antibody’s specificity, a competition assay was performed using a Article Title: The Sarcoplasmic/Endoplasmic reticulum Ca 2+ -ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation Article Snippet: Thereafter, membranes were washed thrice in 1× TBS-Tween 20, and incubated with a secondary goat anti-rabbit antibody (ref. P0448; Dako; 1:10,000; v: v) in agitation for 1 h. Finally, membranes were washed five times in 1× TBS-Tween 20 and bands were visualized using a chemiluminescent substrate in a G: BOX Chemi XL system (Syngene). .. To confirm the specificity of the primary antibody, a competition assay using a Recombinant:Article Title: The Sarcoplasmic/Endoplasmic reticulum Ca 2+ -ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation. Article Snippet: Thereafter, membranes were washed thrice in 1× TBS-Tween 20, and incubated with a secondary goat anti-rabbit antibody (ref. P0448; Dako; 1:10,000; v: v) in agitation for 1 h. Finally, membranes were washed five times in 1× TBS-Tween 20 and bands were visualized using a chemiluminescent substrate in a G: BOX Chemi XL system (Syngene). .. To confirm the specificity of the primary antibody, a competition assay using a Article Title: The Sarcoplasmic/Endoplasmic reticulum Ca 2+ -ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation Article Snippet: Samples were observed under a confocal laser-scanning microscope (CLSM, Nikon A1R; Nikon Corp., Tokyo, Japan) with preset acquisition settings, and the images captured were analyzed with the Fiji ImageJ software . .. To confirm the primary antibody’s specificity, a competition assay was performed using a Article Title: The Sarcoplasmic/Endoplasmic reticulum Ca 2+ -ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation. Article Snippet: Samples were observed under a confocal laser-scanning microscope (CLSM, Nikon A1R; Nikon Corp., Tokyo, Japan) with preset acquisition settings, and the images captured were analyzed with the Fiji ImageJ software57. .. To confirm the primary antibody’s specificity, a competition assay was performed using a Article Title: The Sarcoplasmic/Endoplasmic reticulum Ca 2+ -ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation Article Snippet: Thereafter, membranes were washed thrice in 1× TBS-Tween 20, and incubated with a secondary goat anti-rabbit antibody (ref. P0448; Dako; 1:10,000; v: v) in agitation for 1 h. Finally, membranes were washed five times in 1× TBS-Tween 20 and bands were visualized using a chemiluminescent substrate in a G: BOX Chemi XL system (Syngene). .. To confirm the specificity of the primary antibody, a competition assay using a |